A Rapid, Green High Performance Anion-Exchange Chromatographic Assay for Saccharin and Its Determination in Environmental Water Samples
نویسنده
چکیده
A green, rapid and sensitive high performance anion-exchange chromatographic (HPAEC) method for the determination of saccharin in environmental water samples is reported. A linear range of 5 μg/L to 28.2 μg/mL was found with a theoretical limit of detection of 5 μg/L based on a signal-to-noise ratio of three. No interferences were seen for sodium cyclamate, acesulfame K, aspartame, nitrite, nitrate and chlorate. Following a simple filtration sample pre-treatment a percentage recovery of 92.9 % and an associated coefficient of variation of 3.8 % was found for a sample fortified with 28.2 ng/mL saccharin. Separation was achieved within 2.5 minutes using a SAP 100 mm × 4.6 mm, 8 μm strong anion exchange analytical column with a mobile phase of 0.1 M NaCl at a flow rate of 1.5 mL/min. The develop method avoids the use of potentially environmentally hazardous reagents and minimises the production of waste.
منابع مشابه
A High-Performance Liquid Chromatographic Assay for the Determination of Losartan in Plasma
A rapid and sensitive HPLC method was developed for determination of losartan in plasma. Losartan was extracted from plasma by a two-step extraction procedure using chloroform as extracting solvent in acidic medium. HPLC analysis was performed on a cyano reversed-phase column using phosphate buffer (pH 4.3), acetonitrile (750:250, v/v) as mobile phase with a flow rate of 0.9 mL/min. Sodium dicl...
متن کاملRapid High Performance Liquid Chromatographic Determination of Risperidone in Human Plasma
A simple, rapid and sensitive high-performance liquid chromatographic (HPLC) method for the determination of risperidone in human plasma was developed. An HPLC system based on a Nucleosil C8 column (150?4 mm) and a UV detector (?= 280 nm) were used. A mixture of sodium dihydrogen phosphate buffer-acetonitrile (55:45, v/v) adjusted to pH 6.0 at a flow rate of 1.5 ml min-1 was used as mobile phas...
متن کاملRapid High Performance Liquid Chromatographic Determination of Risperidone in Human Plasma
A simple, rapid and sensitive high-performance liquid chromatographic (HPLC) method for the determination of risperidone in human plasma was developed. An HPLC system based on a Nucleosil C8 column (150?4 mm) and a UV detector (?= 280 nm) were used. A mixture of sodium dihydrogen phosphate buffer-acetonitrile (55:45, v/v) adjusted to pH 6.0 at a flow rate of 1.5 ml min-1 was used as mobile phas...
متن کاملSimple High-Performance Liquid Chromatographic Method for Determination of Ciprofloxacin in Human Plasma
A rapid, simple and sensitive high-performance liquid chromatography method was developed for determination of ciprofloxacin in plasma by means of ultraviolet detection. Ofloxacin was used as an internal standard and separation carried on a Novapak C18 column using a mobile phase of 0.01 M phosphate buffer (pH =2.6): methanol (82: 18 v/v). Extraction of drug was performed from plasma by liquid-...
متن کاملThe Preconcentration and Determination of Pb2+, Cu2+, Co2+, Ni2+, Fe2+ and Zn2+ Elements in Water Samples Using Citrate Form of Anion-Exchange Resin
Lead, copper, cobalt, nickel, iron and zinc ions form monocitrate complexes (negative charge) in citrate solution which is absorbed on the citrate form of anion-exchange resins (citrate as counter ion) y exchange of citrate ion with citrate complexes. These are subsequently recovered from the resin by 0.5M ammonium citrate eluent at pH 2.0. Under optimized conditions, quantitative recovery ...
متن کامل